The virologists from the University of Barcelona have found traces of the novel coronavirus in a sample of Barcelona wastewater dating back in March 2019, which is nine months before the COVID-19 disease got identified in China. Recent studies have suggested that it was circulating in Italy as early as December 2019. All the samples before this date were negative, except for a sample from March 12, 2019, which gave a positive result in their PCR test for coronavirus. "We expect to build upon this work by testing many other kinase inhibitors while concurrently conducting experiments with other technologies to identify underlying pathways and additional potential therapeutics that may intervene in COVID-19 effectively".
One of the early findings about SARS-CoV-2 is that it is found in the feces of infected people.
The virus has spread virtually all over the world, infecting more than 9 million people and claiming nearly 500,000 lives.
They found evidence of the virus on January 15, 2020, 41 days before the first official case was declared on February 25, 2020.More news: Coronavirus Lockdown: Europe Reopens Borders to 15 Countries, US Excluded from List
PCR involves getting samples of saliva, mucus, frozen wastewater or whatever else the virus is thought to be lurking in, clearing all the unnecessary stuff out of the sample, then converting the RNA-which is a single strand of genetic material-into DNA (the famous double-stranded helix). These results encouraged the researchers to analyze frozen samples taken between January 2018 and December 2019.
"The virus prevents human cells from dividing, maintaining them at a particular point in the cell cycle".
Testing for coronavirus is now widely available across Australia. In this case, the researchers tested for three.
The scientists used mass spectrometry, a tool to analyse the properties of a sample by measuring the mass of its molecules and molecular fragments, to evaluate all host and viral proteins that showed changes in phosphorylation after SARS-CoV-2 infection. The identified epitopes can guide the design of vaccines for SARS-CoV-2, as they represent parts of the virus for which a robust, protective immune response may be mounted. "In fact, we have done it for other viruses before and that is why we looked for SARS-CoV-2 in wastewater", Bosch told Xinhua. This sometimes happens in labs as positive samples are regularly being handled, and it can be hard to prevent very small traces of positive sample contaminating others.More news: White House aware of Russian bounties in 2019
Further analysis by first author Mehdi Bouhaddou, PhD, a postdoctoral researcher in Krogan's Lab - and in collaboration with Danielle Swaney at the Gladstone Institutes and Pedro Beltrao at the European Molecular Biology Laboratory's European Biosciences Institute in in Cambridge, England - revealed that 49 kinases exhibited abnormal activity in infected cells.
Both community-based and large-scale serology surveillance efforts-such as the RESPONSE study sponsored by NIAID and NHLBI-are collecting critical data to improve epidemiological models and inform public health decision-making.
The scientists also identified the kinases that are most likely to regulate these modifications, and suggested that these enzymes are potential targets for drugs to stop the activity of the virus and treat COVID-19.
These structures may help the virus reach nearby cells in the body and advance the infection, but further study is warranted.More news: Supreme Court was right to throw out Louisiana's abortion law