The cards sparked outrage on social media with prompts like "Can't you see, the Jews are behind everything - the banks, the media, even _!" and "What's so important right now that you can't call your mother?"
The pack and a similar "Jew Pack", which were released over a year ago, were still available online at other retailers, including the Cards Against Humanity website.
WABC-TV reports that Cards Against Humanity co-founder Max Temkin's grandfather was a Jewish WWII veteran who "was shot down over Germany during a combat mission in World War II and interned in a POW camp".More news: Turkey and Russia finalize deal on anti-missile defense system
The point of the game is to choose an answer to a "fill-in-the-blank" card - the victor of each round is the person who has chosen the most shocking and offensive answer card.
Target did not say how numerous games had been sold or stocked in its stores.
Target quickly responded, offering an apology and plans to remove the extension pack from their stores.More news: Sharks Die of Apparent Cold Shock At Cape Cod
"We are aware of this extended card pack of the game Cards Against Humanity and are in the process of removing it from our stores", the company on Twitter said.
Just when you thought Cards of Humanity couldn't get anymore controversial, American shop Target has had to apologise after complaints surfaced about a particularly indelicate expansion pack - an anti-semitic one.
Some are also pointing out that the games' creators are actually Jewish themselves.More news: OnePlus 5T might get a Sandstone variant in January
The retailer apologised saying "it is never our intention to offend our guests with the products we carry".