The Underground network has been hugely reduced by Transport for London (TfL) with 40 stations shut and many services cut back.
UK Health Secretary Matt Hancock had earlier on March 24, said at a press briefing, that the Mayor should elevate the number of tubes in the underground network that would allow the public to adhere to government's COVID-19 containment measure.
"We should have more tube trains running", he said at a news conference.
"And there is no good reason in the information that I've seen that the current levels of Tube provision should be as low as they are".More news: NASA’s Curiosity Takes New Selfie After Setting Record On Mars
His comments came after commuter trains were still packed on Monday morning, with pictures showing little space between commuters.
"TfL will do everything possible to continue safely running a basic service for key workers, including our incredible NHS staff, but if the number of TfL staff off sick or self-isolating continues to rise - as we sadly expect it will - we will have no choice but to reduce services further".
He added: "We strongly urge the rest of the public to do the right thing and help us save lives by staying at home and slowing the spread of the virus". He lashed out saying that the Transport for London (TFL) was operating the maximum of tube services it can while maintaining safety, however, people weren't following the rules. "We need more people to stay at home and more trains running in morning and evening peak".
But a spokesman for London Mayor Mr Khan insisted ministers have been told "countless times" that TfL can not run a full service due to levels of staff sickness and self-isolation.More news: 2020 iPad Pro gets benchmarked
"Nearly a third of TfL's staff are now off sick or self-isolating - including train drivers and crucial control centre staff".
The mayor added that "too numerous people using TfL services at the busiest times work in construction", as he reiterated his call for the Government to ban non-safety construction work.
He has suggested to the government that it provides a "proper package of support" for these workers so that they can stay in isolation at home.
In a televised address to the nation last night, the prime minister urged people to stay at home unless they had good reason.More news: 3 fresh cases take TN Covid-19 total to 15
Boris Johnson said the lockdown will be enforced by police, and warned those ignoring them would be fined.