As he walked out of the room, a reporter asked the President's son if he was "worried about perjury" following this testimony.
Ultimately, the panel struck a deal with Trump Jr. for him to testify for two-to-four hours on roughly a half-dozen topics.
Special Counsel Robert S. Mueller III's report on Russian interference in the 2016 USA presidential election is believed to be the impetus for the request for a second interview.
Both issues highlighted questions about whether the Trump campaign colluded with Russians in their interference in the election.More news: MPs may have run out of options to block no-deal Brexit
Trump Jr. now heads the Trump Organisation with his younger brother Eric, and his interview is expected to cover a wide array of subjects.
President Donald Trump's eldest son will testify Wednesday behind closed doors to a Republican-led Senate committee investigating Russian Federation election meddling, a congressional source said.
The Russia project never came to fruition but is considered significant because it shows the president and his company were chasing a lucrative business deal in Moscow while the Russian government, according to USA intelligence agencies, was conducting a hacking and propaganda campaign to boost his candidacy.
According to United States intelligence agencies, this happened while the Russian government was conducting a hacking and propaganda campaign to boost Mr Trump's candidacy. Senators want to discuss answers Trump Jr. gave to Congress in 2017 after President Donald Trump's former lawyer, Michael Cohen, told a House committee he had regularly briefed Trump Jr. on a real estate project in Moscow.More news: Apple's iCloud for Windows App Gets a New Version in Microsoft Store
Mr Cohen is now servicing a prison sentence after pleading guilty in two federal cases.
The report is believed to have renewed the committee's interest in a June 2016 Trump Tower meeting attended by Trump Jr., Paul Manafort, Jared Kushner and Natalia Veselnitskaya, a Kremlin-connected Russian attorney who allegedly offered to provide damaging information about then-presidential candidate Hillary Clinton.
The first time Trump Jr. testified before the Senate Intelligence Committee, he was questioned by committee staff with no senators present, which was the committee's protocol for its first round of Russian Federation investigation interviews. The subpoena was criticized by some of the president's most vocal defenders within his Republican Party.More news: Elon Musk: Tesla is not dealing with a demand problem
Trump initially denied he had any business links to Russian Federation at the time.