Hugo Carvajal, 59, was arrested by Spanish police at 3:30pm local time on Friday, on the request of United States authorities, who have been trying to detain him on drug trafficking charges for many years.
Carvajal, dubbed "El Pollo" (The Chicken), was taken into custody Friday at his son's home in Madrid by Spanish police.
A spokeswoman with Spain's National Court, which handles extradition cases, said that Carvajal would testify on Saturday before a judge in Madrid.
Carvajal denies the drug trafficking charges and is set to fight the United States extradition request.More news: IMF Officials Urge Governments to Limit Risks to Global Economy
He refused to be handed over to USA officials and argued that he now has family ties to Spain.
While Spanish police did not fully disclose the charges brought against the general, in 2008 he was sanctioned by the US Treasury for "materially assisting the narcotics trafficking activities" of Colombia's FARC rebel group.
Carvajal, who climbed the ranks in Venezuela since he befriended Chvez in the early 1980's, is no stranger to U.S. law enforcement agencies.
Carvajal has always been sought by US Treasury officials who suspect him of providing support to drug trafficking by FARC guerrillas.More news: Honor 20 series to be announced on May 21
In 2014, Carvajal was detained at the request of the United States in Aruba, the Netherlands' island in the Caribbean, where he served as a Venezuelan consul general.
During the hearing, Carvajal denied the accusations by U.S. prosecutors and challenged his potential extradition, a court spokesman said.
On February 21, he posted a video on Twitter in which he told army officers: "How it all ends will depend on you my brothers in arms" and urging them to stand "on the right side of history". Guaido was later recognized by the United States and dozens of governments, but Maduro remains in office with support of the military and has denounced Guaido as a USA puppet.More news: Here's the first trailer for season two of Big Little Lies