Perhaps the most poignant line in their announcement is this: "If we had known we would separate after 25 years, we would do it all again".
According to Bezos and MacKenzie, the two will "continue [their] shared lives as friends". Some have run the numbers to find out that if Bezos were to split his eye-watering $137 billion fortune with his wife, it could make her the wealthiest woman in the world. So who is MacKenzie Bezos, and what will the couples' divorce likely entail?
Bezos and MacKenzie announced in a statement on the billionaire's Twitter account that they had chose to continue their "shared lives as friends". The couple has four kids, with one of them adopted from China.
Amazon has since evolved from an upstart website selling books to an e-commerce goliath that sells virtually all imaginable merchandise and runs data centers that power many other digital services such as Netflix.More news: Sanders Apologizes Amid New Allegation of Staff Harassment
Last year, the pair launched the Bezos Day One Fund, offering a $2bn (€1.73bn) fund to help homeless families and build pre-schools in low-income neighbourhoods.
Jeff Bezos also founded space company Blue Origin in 2000, and is funneling more than $1 billion a year of his own fortune into pulling it out of start-up mode and into production. They got married just six months after they began dating, according to Bezos.
The pair originally met while they were both working at a hedge fund in NY.
MacKenzie Bezos performed accounting for Amazon in the first year of its existence, but reportedly does not hold any shares directly. They had already been through a trial separation in private.
He had solicited ideas on Twitter in 2017 for ways to donate some of his wealth.More news: MCTS bus driver saves 1-year-old left alone on overpass
In 2012, they donated $2.5 million to a Washington state campaign to legalize same-sex nuptials there.
From modest beginnings as an online bookseller, Mr Bezos and Amazon branched out into nearly every product category available, ending up taking on established retail giants such as Walmart. Also, a $13 billion purchase of Whole Foods is helping the company expand deeper into groceries.
He also owns The Washington Post, which has been a target of criticism from US President Donald Trump.
Billionaire Jeff Bezos' divorce from his wife MacKenzie Bezos could become the most expensive in history and has the potential to impact his ownership and control of Amazon if he is forced to sell off shares to fund a settlement.More news: FDA recalls candy over possible hepatitis A contamination